Fluorescent biosensors for multiplexing G protein-coupled receptors (GPCR) functional assays
A Basque biotechnology SME focused on developing cell-based assays for High-Throughput Screening (HTS) & High- Content Analysis (HCA) has developed a family of fluorescent biosensors which allows the measurement of different G protein-coupled receptor (GPCR) activation pathways (G-Protein & β-arrestin) simultaneously in living cells by fluorescent imaging analysis on most HTS & high-content screening (HCS) platforms. They are looking for commercial with technical assistance or licence agreements
The company wishes to collaborate with pharmaceutical or biotechnology companies, research centres and/or government institutions interested in accelerating and improving their drug discovery programs. They are looking for a commercial agreement with technical assistance or a licensing agreement regarding the use of new fluorescent biosensors assays technology as a highly robust and well suited tool for high throughput screening (HTS) applications.
The company is a dynamic and R&D intensive biotechnological SME located in the Basque Country. They are focused on developing cell-based assays for High-Throughput Screening and High-Content Analysis. They also act as a contract research organization providing drug screening services and on-demand assay development. Their goal is to accelerate and improve their customer’s drug discovery programs. This SME has developed a novel family of fluorescent biosensors for measuring G-protein coupled receptors (GPCR) activity in living cells by means of analysing the different activation pathways simultaneously. Each biosensor of the family co-expressed with a GPCR, provides an innovative and sensitive research tool for studying the molecular mechanism and kinetics of GPCR activation. These biosensors enable the measurement of both, beta-arrestin recruitment and second messenger concentration changes (Ca++, cAMP, DAG) involved in GPCR activation. An increase in the second messenger concentration leads to a change in the structural folding of the green fluorescent biosensor that increases its intensity signal. At the same time, when activated & phosphorylated GPCR binds the beta-arrestin, leads another change in the structural folding of the red fluorescent biosensor, increasing also its fluorescence intensity. Four different versions of biosensors have been actually developed: β-arrestin, cAMP, Ca++ and DAG. The company is looking for a commercial agreement with technical assistance or a licensing agreement with pharma or biotech companies, research centres or government institutions interested in accelerating and improving their drug discovery programs.
Advantages and innovations
The new biosensors technology allows for the performance of screening campaigns measuring both β-arrestin & second messenger concentration changes simultaneously in living cells by fluorescence intensity. It could be applied to GPCRs activation/inhibition studies, primary cells or to orphan receptors because this technology allows performing multiplex assays. The main advantages are: • Performance of multiplex analysis, analysing both GPCR activation pathways • Different second messenger application: cAMP, Ca++, DAG. • β-Arrestin recruitment monitorization • Direct identification of biased ligands • Non-tagged GPCRs. • Economic assay. It is not required to use additional reagents to perform the determinations. The biosensors assays are highly robust and well suited for high throughput screening (HTS) applications. The use of these biosensors in a HTS campaign reduces the time, costs and resources needed in more than a 50-70%.
Already on the market
Intellectual Property Rights (IPR)
Patent(s) applied for but not yet granted,Patents granted
Register your interest
How it works
- Tell us about yourself
- We’ll discuss with you
- We put the right partners in touch
EEN help you find the right partner, rather than you going it alone.
Our role is to review and collate the most suitable submissions, and then send them to the client who posted the opportunity. We consult with you, and the client, to make the process professional and easy.
These are live opportunities. Your registration of interest on the site is just like a professional approach to a business at a networking event. To stand the best chance of success, make your submission really sing. Sell why the client who posted the opportunity should work with you. Excite them. Ask questions. Try and avoid copy and pasting words from elsewhere.
Once the client has chosen their partner, we'll introduce them over email and keep in touch with both parties to see how it's going. Sometimes things progress quickly. Sometimes because of changing priorities for either party, things progress slowly, but you never know - your next big business break could start right here.
First we need to check you’re human.
Thank you for verifying your email. We have sent you a confirmation email containing a 6 digit verification code to unlock the form below